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Docente
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CANDI ELEONORA
(programma)
DESCRIPTION OF TEACHING MODULE:
The course is divided in four parts: maintenance of the genome, genome expression, regulation of genome expression and molecular biology methodologies.
Maintenance of the genome: Structures of DNA and RNA, genome structure, chromatin and nucleosome, replication of DNA, mutability and repair of DNA, homologous recombination at the molecular level site-specific recombination.
Genome expression: Mechanism of transcription, RNA splicing, translation, the genetic code.
Regulation of genome expression: Transcriptional regulation in prokaryotes and in eukaryotes, regulatory RNAs.
Methods: DNA and RNA electrophoresis, hybridization, restriction enzymes and cloning techniques, oligonucleotides synthesis, PCR, EMSA, chromatin immunoprecipitation, DNA sequencing, gene array.
 DIDACTIC MATERIALS:
1) Text book: Molecular Biology of the Gene, Watson, Baker, Bell, Gann, Levine, Losick.
Pearson International Edition.
2) Scientific seminars on different molecular biology topics organized every year in which the students are invited.
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